forサイクル出力の末尾を取り除き、

person_info = [{"name":"yuze", "age": '88', "gender": " ", "hobby": "hha", "motto": "hehe"},
               {"name":"boge", "age": '18', "gender": " ", "hobby": "basketball", "motto": "haha"}]


for i in person_info:
   print( ",".join([ str(v) for v in i.values()]))