python opencvは画像の配合と比較を実現します。

from skimage import io
import cv2 as cv
import numpy as np
import matplotlib.pyplot as plt
 
img_path1 = '2_HE_maxarea.png'
img_path2 = '2_IHC_maxarea.png'
 
img1 = io.imread(img_path1)
img2 = io.imread(img_path2)
img1 = np.uint8(img1)
img2 = np.uint8(img2)
 
# find the keypoints and descriptors with ORB
orb = cv.ORB_create()
kp1, des1 = orb.detectAndCompute(img1,None)
kp2, des2 = orb.detectAndCompute(img2,None)
 
# def get_good_match(des1,des2):
#  bf = cv.BFMatcher()
#  matches = bf.knnMatch(des1, des2, k=2)
#  good = []
#  for m, n in matches:
#   if m.distance < 0.75 * n.distance:
#    good.append(m)
#  return good,matches
# goodMatch,matches = get_good_match(des1,des2)
# img3 = cv.drawMatchesKnn(img1,kp1,img2,kp2,matches[:20],None,flags=2)
 
# create BFMatcher object
bf = cv.BFMatcher(cv.NORM_HAMMING, crossCheck=True)
# Match descriptors.
matches = bf.match(des1,des2)
# Sort them in the order of their distance.
matches = sorted(matches, key = lambda x:x.distance)
# Draw first 20 matches.
img3 = cv.drawMatches(img1,kp1,img2,kp2,matches[:20],None, flags=2)
 
 
goodMatch = matches[:20]
if len(goodMatch) > 4:
 ptsA= np.float32([kp1[m.queryIdx].pt for m in goodMatch]).reshape(-1, 1, 2)
 ptsB = np.float32([kp2[m.trainIdx].pt for m in goodMatch]).reshape(-1, 1, 2)
 ransacReprojThreshold = 4
 H, status =cv.findHomography(ptsA,ptsB,cv.RANSAC,ransacReprojThreshold);
 #  H           
 #status                  。
 #ptsA,ptsB    
 #cv2.RANSAC, ransacReprojThreshold      RANSAC  
 imgOut = cv.warpPerspective(img2, H, (img1.shape[1],img1.shape[0]),flags=cv.INTER_LINEAR + cv.WARP_INVERSE_MAP)
 
#            
rate = 0.5
overlapping = cv.addWeighted(img1, rate, imgOut, 1-rate, 0)
io.imsave('HE_2_IHC.png', overlapping)
err = cv.absdiff(img1,imgOut) 
 
#     
plt.subplot(221)
plt.title('orb')
plt.imshow(img3)
 
plt.subplot(222)
plt.title('imgOut')
plt.imshow(imgOut)
 
plt.subplot(223)
plt.title('overlapping')
plt.imshow(overlapping)
 
plt.subplot(224)  
plt.title('diff') 
plt.imshow(err)
 
plt.show()